SDF4 anticorps (AA 161-175)

N° du produit ABIN625824

Cet anticorps anti-SDF4 est un anticorps Chèvre Polyclonal détectant SDF4 dans WB, ELISA, IHC, FACS et IF. Adapté pour Humain.

Aperçu rapide pour SDF4 anticorps (AA 161-175) (ABIN625824)

Antigène: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SDF4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-SDF4 anticorps

Épitope: AA 161-175

Fonction: SDF4 (aa161-175)

Séquence: EYKVKFLASK GHSEK

Specificité: This antibody is expected to recognize both reported isoforms NP_057631.2, and NP_057260.3

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: CEYKVKFLASKGHSEK

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8 kDa according to NP_057260.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SDF4

Antigène: SDF4 (Stromal Cell Derived Factor 4 (SDF4))

Autre désignation: SDF4

Sujet: SDF4, stromal cell derived factor 4, Cab45, 45 kDa calcium-binding protein, OTTHUMP00000001754, SDF-4, calcium binding protein, stromal cell-derived factor 4

ID gène: 51150, 20318, 155173

NCBI Accession: NP_057631, NP_057260