USP28 anticorps (N-Term)

N° du produit ABIN6258349

Détail du produit anti-USP28 anticorps

Antigène: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Épitope: N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp USP28 est non-conjugé

Application: Western Blotting (WB), ELISA

Specificité: USP28 Antibody detects endogenous levels of total USP28.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human USP28, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Date de péremption: 12 months

Détails sur USP28

Antigène: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Autre désignation: USP28 (USP28 Produits)

Synonymes: anticorps im:7138195, anticorps USP28, anticorps 9830148O20Rik, anticorps AU022237, anticorps mKIAA1515, anticorps ubiquitin specific peptidase 28, anticorps USP28, anticorps usp28, anticorps Usp28

Sujet:

Description: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).

Gene: USP28

Poids moléculaire: 122 kDa

ID gène: 57646

UniProt: Q96RU2