OCT4 anticorps (AA 114-163)

N° du produit ABIN625854

Cet anticorps anti-OCT4 est un anticorps Lapin Polyclonal détectant OCT4 dans WB, IHC et IHC (p). Adapté pour Humain, Porc, Lapin et Chévre.

Aperçu rapide pour OCT4 anticorps (AA 114-163) (ABIN625854)

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivité: Humain, Porc, Lapin, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-OCT4 anticorps

Épitope: AA 114-163

Marque: IHC-plus™

Specificité: Human POU5F1 / OCT4

Homologie: Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).

Purification: Protein A/G purified

Immunogène: Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: PBS, 2 % sucrose.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: POU5F1 / OCT4

Sujet: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

ID gène: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance