OCT4 anticorps (AA 114-163)

N° du produit ABIN625854

Détail du produit anti-OCT4 anticorps

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Épitope: AA 114-163

Reactivité: Humain, Porc, Lapin, Chévre

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp OCT4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human POU5F1 / OCT4

Homologie: Percent identity by BLAST analysis: Human, Goat (100%) Rabbit (92%) Dog (91%) Pig (84%).

Purification: Protein A/G purified

Immunogène: Synthetic peptide located between aa114-163 of human POU5F1 (Q01860, NP_002692). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Goat, Rabbit (100%), Marmoset (92%), Dog (91%), Bovine, Pig, Guinea pig (85%), Horse (84%), Cat (83%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: PBS, 2 % sucrose.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur OCT4

Antigène: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Autre désignation: POU5F1 / OCT4 (POU5F1 Produits)

Synonymes: anticorps OCT3, anticorps OCT4, anticorps OTF-3, anticorps OTF3, anticorps OTF4, anticorps Oct-3, anticorps Oct-4, anticorps POU5F1, anticorps gp9, anticorps gp-9, anticorps oct4, anticorps pou2, anticorps POU-2, anticorps cb197, anticorps spg/pou2, anticorps chunp6868, anticorps wu:fd18d06, anticorps NF-A3, anticorps Oct-3/4, anticorps Oct3, anticorps Oct3/4, anticorps Oct4, anticorps Otf-3, anticorps Otf-4, anticorps Otf3, anticorps Otf3-rs7, anticorps Otf3g, anticorps Otf4, anticorps POU class 5 homeobox 1, anticorps POU domain, class 5, transcription factor 3, anticorps POU domain, class 5, transcription factor 1, anticorps POU5F1, anticorps pou5f3, anticorps Pou5f1

Sujet: Name/Gene ID: POU5F1

Synonyms: POU5F1, Oct-4, OCT3, OCT4, OTF4, POU class 5 homeobox 1, Octamer-binding protein 3, Octamer-binding protein 4, OTF-3, Oct-3, OTF3

ID gène: 5460

NCBI Accession: NP_002692

UniProt: Q01860

Pathways: Stem Cell Maintenance