MHC Class II RT-1B anticorps

N° du produit ABIN625901

L’anticorps Souris Monoclonal anti-MHC Class II RT-1B a été validé pour FACS, IHC (fro), IHC (p), WB et IHC. Il convient pour détecter MHC Class II RT-1B dans des échantillons de Rat et Souris.

Aperçu rapide pour MHC Class II RT-1B anticorps (ABIN625901)

Antigène: MHC Class II RT-1B

Reactivité: Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II RT-1B est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: OX-6

Détail du produit anti-MHC Class II RT-1B anticorps

Marque: IHC-plus™

Specificité: Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

Purification: Protein G purified

Immunogène: Rat thymocyte membrane glycoprotein.

Type of Immunogen: Membrane extract

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer  pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)

Commentaires:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur MHC Class II RT-1B

Antigène: MHC Class II RT-1B