CHMP4B anticorps (N-Term)

N° du produit ABIN6260812

Cet anticorps anti-CHMP4B est un anticorps Lapin Polyclonal détectant CHMP4B dans IHC, WB, IF et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour CHMP4B anticorps (N-Term) (ABIN6260812)

Antigène: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHMP4B est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-CHMP4B anticorps

Épitope: N-Term

Specificité: CHMP4B Antibody detects endogenous levels of total CHMP4B.

Homologie: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human CHMP4B, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur CHMP4B

Antigène: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Autre désignation: CHMP4B

Sujet:

Description: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released (PubMed:12860994, PubMed:18209100). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Gene: CHMP4B

Poids moléculaire: 29-33 kDa

ID gène: 128866

UniProt: Q9H444