Histone H3.3 anticorps (N-Term)

N° du produit ABIN6262140

L’anticorps anti-Histone H3.3 Polyclonal Lapin est utilisé pour la détection de Histone H3.3 dans des échantillons de Humain et Souris. Il a été validé pour WB, IHC, IF et ICC.

Aperçu rapide pour Histone H3.3 anticorps (N-Term) (ABIN6262140)

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H3.3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Histone H3.3 anticorps

Épitope: N-Term

Fonction: H3F3A Antibody

Specificité: H3F3A Antibody detects endogenous levels of total H3F3A.

Homologie: Pig,Zebrafish,Bovine,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human H3F3A, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur Histone H3.3

Antigène: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Autre désignation: H3F3A

Sujet:

Description: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene: H3F3A, H3F3B

Poids moléculaire: 19 kDa

ID gène: 3020, 3021

UniProt: P84243