LC3A / LC3B (N-Term) anticorps

N° du produit ABIN6262925

Cet anticorps Lapin Polyclonal détecte spécifiquement LC3A / LC3B dans IF, WB, ICC et IHC. Il présente une réactivité avec des échantillons de Humain, Rat et Souris. Il a été cité dans 4+ publications.

Aperçu rapide pour LC3A / LC3B (N-Term) anticorps (ABIN6262925)

Antigène: LC3A / LC3B

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Détail du produit

Épitope: N-Term

Fonction: LC3A/B Antibody

Specificité: LC3A/B Antibody detects endogenous levels of total LC3A/B.

Homologie: Pig,Zebrafish,Bovine,Sheep,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human LC3B, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Référence

Tang, Cui, Luan, Ruan, Wang, Yang, Wang, Liu, Wang: "Human tissue kallikrein 1 ameliorates erectile function via modulation of macroautophagy in aged transgenic rats." dans: Andrology, (2018) (PubMed).

Feng, Lu, He, Wang, Qi, Pi, Zhang: "Mycobacterium smegmatis Induces Neurite Outgrowth and Differentiation in an Autophagy-Independent Manner in PC12 and C17.2 Cells." dans: Frontiers in cellular and infection microbiology, Vol. 8, pp. 201, (2018) (PubMed).

Shao, Yang, Xu, Zhu, Huang, Li, Wu, Yue, Xiong, Chen, Kuang, Fan, Zhu, Zeng: "Inhibition of mTORC1 signaling protects kidney from irradiation-induced toxicity via accelerating recovery of renal stem-like cells." dans: Stem cell research & therapy, Vol. 9, Issue 1, pp. 219, (2018) (PubMed).

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." dans: OncoTargets and therapy, Vol. 11, pp. 4501-4510, (2018) (PubMed).

Détail du antigène

Antigène: LC3A / LC3B

Autre désignation: LC3A/B

Sujet:

Description: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene: MAP1LC3B

Poids moléculaire: 14.16 kDa

ID gène: 84557, 81631

UniProt: Q9H492