PUM2 anticorps (N-Term)
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- Antigène Voir toutes PUM2 Anticorps
- PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PUM2 est non-conjugé
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Application
- Western Blotting (WB), ELISA
- Specificité
- PUM2 Antibody detects endogenous levels of total PUM2.
- Homologie
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogène
- A synthesized peptide derived from human PUM2, corresponding to a region within N-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product PUM2 Anticorps primaire
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- Indications d'application
- WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20 °C. Stable for 12 months from date of receipt.
- Date de péremption
- 12 months
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- Antigène
- PUM2 (Pumilio Homolog 2 (Drosophila) (PUM2))
- Autre désignation
- PUM2 (PUM2 Produits)
- Synonymes
- anticorps pum-2, anticorps 5730503J23Rik, anticorps Pumm2, anticorps mKIAA0235, anticorps PUMH2, anticorps PUML2, anticorps pumilio RNA binding family member 2, anticorps pumilio RNA-binding family member 2, anticorps pum2, anticorps Pum2, anticorps PUM2
- Sujet
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Description: Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). May regulate DCUN1D3 mRNA levels (PubMed:25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233).
Gene: PUM2
- Poids moléculaire
- 114 kDa
- ID gène
- 23369
- UniProt
- Q8TB72
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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