Reelin anticorps (N-Term)

N° du produit ABIN6264693

L’anticorps Lapin Polyclonal anti-Reelin a été validé pour WB, IHC et ELISA et a été validé indépendamment. Il convient pour détecter Reelin dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Reelin anticorps (N-Term) (ABIN6264693)

Antigène: Reelin (RELN)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Reelin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-Reelin anticorps

Épitope: N-Term

Specificité: RELN Antibody detects endogenous levels of total RELN.

Homologie: Pig,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human RELN, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104518 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104518

Date: 26.01.2024

Antigène: Reelin

Numéro du lot: 10M5728

Application validée: Immunohistochemistry

Contrôle positif:

Postnatal day 5 and adult (> 2 months) CD1 mouse brain fixed in 4% paraformaldehyde.

Contrôle négative:

One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.

Conclusion:

Passed. The Reelin antibody (AA 491-590) ABIN6264693 works in IHC-P at 1:50 concentrations with microwave antigen retrieval.

Validation Images

Reelin expression in the postnatal day 5 mouse cerebellum. EGL = external granular layer of the cerebellar cortex; IGL = internal granular layer of the cerebellar cortex;PCL/ML = Purkinje cell/molecular layers of the cerebellar cortex; WM = white matter. Staining is mainly observed in the processes of the pre-migratory granule cells. Original magnifications: A=10x; B= 20x

Protocole

Anticorps primaire: ABIN6264693

Anticorps secondaire: anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A11037, lot # 2439060)

Full Protocol:

• Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix of samples in the same fixative for an additional 2 h at RT.
• Wash, dehydrate, and embed samples in paraffin wax.
• Wash several times with 0.01 M PBS.
• Cut brain with a microtome into 6 µm sections and mount on glass slides.
• After paraffin removal processed sections for microwave antigen retrieval for 10 minutes (95-100° C) in 10 mM sodium citrate buffer (pH 6.0). After 20 minutes of spontaneous cooling, wash sections (distilled water, 2 times for 5 minutes each; followed by PBS for 5 minutes).
• Incubate sections for 1 h at RT in a humid chamber in PBS containing 1% ovalbumin (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
• Incubate sections with primary rabbit anti-Reelin (antibodies-online, ABIN6264693, lot 10M5728) diluted 1:50, in PBS-BSA-PLL ON at RT in a humid chamber.
• Wash sections 3x 5 min with 0.01 M PBS.
• Incubate sections with anti-rabbit secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A11037, lot # 2439060), 1:500, in 0.1M PBS, 1 hour at room temperature.
• Wash sections 3x 5 min with 0.01 M PBS.
• Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
• Acquire images Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur Reelin

Antigène: Reelin (RELN)

Autre désignation: RELN

Sujet:

Description: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation (By similarity).

Gene: RELN

Poids moléculaire: 388kDa

ID gène: 5649

UniProt: P78509

Pathways: Synaptic Membrane