WT1 anticorps (N-Term)

N° du produit ABIN6266054

Cet anticorps Lapin Polyclonal détecte spécifiquement WT1 dans IHC, IF, WB, ELISA et ICC. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour WT1 anticorps (N-Term) (ABIN6266054)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WT1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-WT1 anticorps

Épitope: N-Term

Specificité: WT1 Antibody detects endogenous levels of total WT1.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human WT1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: WT1

Sujet:

Description: Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Gene: WT1

Poids moléculaire: 49kDa

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation