PIWIL4 anticorps (N-Term)

N° du produit ABIN6266377

Détail du produit anti-PIWIL4 anticorps

Antigène: PIWIL4 (Piwi-Like 4 (PIWIL4))

Épitope: N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIWIL4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: PIWIL4 Antibody detects endogenous levels of total PIWIL4.

Homologie: Horse

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human PIWIL4, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur PIWIL4

Antigène: PIWIL4 (Piwi-Like 4 (PIWIL4))

Autre désignation: PIWIL4 (PIWIL4 Produits)

Synonymes: anticorps HIWI2, anticorps MIWI2, anticorps 9230101H05Rik, anticorps Miwi2, anticorps piwi like RNA-mediated gene silencing 4, anticorps piwi-like RNA-mediated gene silencing 4, anticorps PIWIL4, anticorps Piwil4

Sujet:

Description: Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity). Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity). The piRNA process acts upstream of known mediators of DNA methylation (By similarity). Does not show endonuclease activity (By similarity). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373).

Gene: PIWIL4

Poids moléculaire: 97kDa

ID gène: 143689

UniProt: Q7Z3Z4