WT1 anticorps (AA 1-181)

N° du produit ABIN6294230

L’anticorps Souris Monoclonal anti-WT1 a été validé pour IHC, IF et FACS. Il convient pour détecter WT1 dans des échantillons de Humain.

Aperçu rapide pour WT1 anticorps (AA 1-181) (ABIN6294230)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp WT1 est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-WT1 anticorps

Épitope: AA 1-181

Fonction: Mouse anti-Human Wilms Tumor 1 Antibody [Sodium Azide Free]

Specificité: Cytoplasmic, membranous

Purification: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Immunogène: A recombinant protein corresponding to residues 1-181 from the human protein was used as the immunogen for the Wilms Tumor 1 antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur WT1

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: Wilms tumor protein

Sujet:

Target Description: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Gene Symbol: WT1

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation