Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) anticorps

N° du produit ABIN6294442

Cet anticorps Souris Monoclonal détecte spécifiquement Isocitrate Dehydrogenase [NADP],cytoplasmic dans IHC, FACS, IF et WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2) (AA 280-420) anticorps (ABIN6294442)

Antigène: Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Détail du produit

Épitope: AA 280-420

Fonction: Mouse anti-Human IDH1 Antibody [Sodium Azide Free]

Specificité: Cytoplasmic & nuclear

Purification: A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.

Immunogène: A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détail du antigène

Antigène: Isocitrate Dehydrogenase [NADP],cytoplasmic (IDH2)

Autre désignation: Isocitrate dehydrogenase cytoplasmic

Sujet:

Target Description: It recognizes a 45 kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming a-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of a-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.

Gene Symbol: IDH1///PICD

ID gène: 3417

UniProt: O75874