NEFH anticorps

N° du produit ABIN6296241

Cet anticorps anti-NEFH est un anticorps Souris Monoclonal détectant NEFH dans IHC et FACS. Adapté pour Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille et Cat.

Aperçu rapide pour NEFH anticorps (ABIN6296241)

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivité: Humain, Rat, Souris, Poulet, Porc, Boeuf (Vache), Lapin, Cobaye, Gerbille, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NEFH est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-NEFH anticorps

Fonction: Mouse anti-Human/Mouse/Rat/Guinea pig/Gerbil/Cat/Pig/Rabbit/Cow/Chicken NF-H Antibody (phospho) [Sodium Azide Free]

Specificité: Cytoplasmic

Purification: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Immunogène: Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the NF-H antibody (phospho).

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur NEFH

Antigène: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Autre désignation: Neurofilament heavy polypeptide

Sujet:

Target Description: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

Gene Symbol: NEFH///KIAA0845///NFH

ID gène: 4744

UniProt: P12036