Vimentin anticorps

N° du produit ABIN6296593

Cet anticorps Souris Monoclonal détecte spécifiquement Vimentin dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat et Chévre.

Aperçu rapide pour Vimentin anticorps (ABIN6296593)

Antigène: Vimentin (VIM)

Reactivité: Humain, Porc, Boeuf (Vache), Poulet, Chien, Cat, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-Vimentin anticorps

Fonction: Mouse anti-Human/Cow/Dog/Cat/Pig/Goat/Chicken VIM Antibody [Sodium Azide Free]

Specificité: Cytoplasmic

Purification: Human recombinant protein was used as the immunogen for this VIM antibody.

Immunogène: Human recombinant protein was used as the immunogen for this VIM antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet:

Target Description: This mAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Gene Symbol: VIM

ID gène: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis