IGLV1-51 anticorps

N° du produit ABIN6296641

Détail du produit anti-IGLV1-51 anticorps

Antigène: IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IGLV1-51 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Fonction: Mouse anti-Human Lambda Light Chain Antibody [Sodium Azide Free]

Specificité: Cell Surface, Cytoplasmic and Secreted

Purification: IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.

Immunogène: IgG purified from human serum was used as the immunogen for this Lambda Light Chain antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur IGLV1-51

Antigène: IGLV1-51 (Immunoglobulin lambda Variable 1-51 (IGLV1-51))

Autre désignation: Immunoglobulin lambda variable 1-51 (IGLV1-51 Produits)

Synonymes: anticorps IGLV151, anticorps V1-19, anticorps immunoglobulin lambda variable 1-51, anticorps IGLV1-51

Sujet:

Target Description: Antibodies are produced by B lymphocytes, each expressing only one class of light chain. Once set, light chain class remains fixed for the life of the B lymphocyte. In a healthy individual, the total kappa to lambda ratio is roughly 3:1 in serum (measuring intact whole antibodies) or 1:1.5 if measuring free light chains, with a highly divergent ratio indicative of neoplasm.Individual B-cells in lymphoid tissue possess either kappa or lambda light chains, but never both together. Specific rearrangement of lambda light chain of immunoglobulins can lead to loss of some protein coding genes, which does not seem to be functionally relevant (while functionally relevant miR-650 can be overexpressed). Using immunohistochemistry, it is possible to determine the relative abundance of B-cells expressing kappa and lambda light chains. If the lymph node or similar tissue is reactive, or otherwise benign, it should possess a mixture of kappa positive and lambda positive cells. If, however, one type of light chain is significantly more common than the other, the cells are likely all derived from a small clonal population, which may indicate a malignant condition, such as B-cell lymphoma. [Wiki]

Gene Symbol: IGLV1-51///IGLC1

ID gène: 3535

UniProt: P01701, P0CG04