ICAM-3/CD50 anticorps

N° du produit ABIN6297692

L’anticorps Souris Monoclonal anti-ICAM-3/CD50 a été validé pour FACS, IF et IHC. Il convient pour détecter ICAM-3/CD50 dans des échantillons de Humain.

Aperçu rapide pour ICAM-3/CD50 anticorps (ABIN6297692)

Antigène: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ICAM-3/CD50 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Détail du produit anti-ICAM-3/CD50 anticorps

Fonction: Mouse anti-Human ICAM-3 Antibody [Sodium Azide Free]

Specificité: Cell surface

Purification: Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

Immunogène: Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur ICAM-3/CD50

Antigène: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Autre désignation: Intercellular adhesion molecule 3

Sujet:

Target Description: Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V). Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.

Gene Symbol: ICAM3

ID gène: 3385

UniProt: P32942