Golgi anticorps

N° du produit ABIN6297710

Détail du produit

Antigène: Golgi

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Fonction: Mouse anti-Human Golgi Antibody [Sodium Azide Free]

Specificité: Golgi complex in cytoplasm

Purification: SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

Immunogène: SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

Information d'application

Indications d'application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml)
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 μg/mL for 30 min
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détail du antigène

Antigène: Golgi

Sujet:

Target Description: This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.