STRO-1 anticorps

N° du produit ABIN638439

Cet anticorps anti- est un anticorps Souris Polyclonal détectant dans FACS et ICC. Adapté pour Humain, Lapin et Rat. Ce Primary Antibody a été cité dans 7+ publications.

Aperçu rapide pour STRO-1 anticorps (ABIN638439)

Antigène: STRO-1

Reactivité: Humain, Lapin, Rat

Hôte: Souris

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit

Fonction: Anti-Hu STRO-1 Purified

Specificité: The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.

Réactivité croisée (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Human CD34 positive bone marrow cells

Isotype: IgM lambda

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Kaneko, Akita, Shimauchi, Sasano: "Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy." dans: Journal of electron microscopy, Vol. 58, Issue 6, pp. 363-73, (2009) (PubMed).

Seo, Miura, Sonoyama, Coppe, Stanyon, Shi: "Recovery of stem cells from cryopreserved periodontal ligament." dans: Journal of dental research, Vol. 84, Issue 10, pp. 907-12, (2005) (PubMed).

Bensidhoum, Chapel, Francois, Demarquay, Mazurier, Fouillard, Bouchet, Bertho, Gourmelon, Aigueperse, Charbord, Gorin, Thierry, Lopez: "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment." dans: Blood, Vol. 103, Issue 9, pp. 3313-9, (2004) (PubMed).

Gronthos, Zannettino, Hay, Shi, Graves, Kortesidis, Simmons: "Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow." dans: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1827-35, (2003) (PubMed).

Stenderup, Justesen, Eriksen, Rattan, Kassem: "Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 16, Issue 6, pp. 1120-9, (2001) (PubMed).

Oyajobi, Lomri, Hott, Marie: "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody." dans: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 14, Issue 3, pp. 351-61, (1999) (PubMed).

Simmons, Torok-Storb: "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1." dans: Blood, Vol. 78, Issue 1, pp. 55-62, (1991) (PubMed).

Détail du antigène

Antigène: STRO-1

Sujet: STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10 % of mononuclear cells, greater than 95 % of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.,STRO1