His Tag anticorps
Aperçu rapide pour His Tag anticorps (ABIN652278)
Antigène
Voir toutes His Tag AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Purification
- This antibody is purified through a protein A column, followed by peptide affinity purification.
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Immunogène
- Poly-HIS peptide were used to produced this antibody.
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Isotype
- Ig Fraction
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Indications d'application
- WB: 1:8000. WB: 1:1000. WB: 1:1000
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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Date de péremption
- 6 months
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: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." dans: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
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- His Tag
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Autre désignation
- 6 X His Tag
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Classe de substances
- Tag
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Sujet
- Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.
Antigène
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