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His Tag anticorps

Name: His Tag anticorps
SKU: ABIN652278
Price: 475.5 EUR
Availability: InStock

Reactivité: Humain WB Hôte: Lapin Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane. (His Tag anticorps)

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane. (His Tag anticorps)

2 images

(1 reference)

N° du produit ABIN652278

Fiche de données

Antigène Voir toutes His Tag Anticorps

His Tag
Reactivité
18

4

4

1

1

1
Humain
Hôte
94

55

12

9

8

7

2

1
Lapin
Anticorps secondaires appropriés
Clonalité
107

68

10
Polyclonal
Conjugué
90

23

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp His Tag est non-conjugé
Application
152

103

38

28

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB)

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène

Poly-HIS peptide were used to produced this antibody.

Clone

RB19960

Isotype

Ig Fraction

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anti-His Tag antibody
WB, ELISA Hôte: Lapin Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IHC, FACS Hôte: Souris Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Hôte: Souris Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
Reactivité: Tag, Humain WB Hôte: Souris Monoclonal 2D5 unconjugated

anti-His Tag antibody (HRP)
WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2 HRP

anti-His Tag (C-Term) antibody
WB, ELISA Hôte: Lapin Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2 Biotin

anti-His Tag (N-Term) antibody (Biotin)
WB, ELISA, IP, FACS, ICC, IHC (p) Hôte: Lapin Monoclonal RM146 Biotin

Indications d'application

WB: 1:8000. WB: 1:1000. WB: 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." dans: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
Antigène

His Tag

Autre désignation

6 X His Tag (His Tag Produits)

Classe de substances

Tag

Sujet

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.