CDT2/RAMP anticorps (AA 229-256)

N° du produit ABIN654926

L’anticorps Lapin Polyclonal anti-CDT2/RAMP a été validé pour WB et FACS. Il convient pour détecter CDT2/RAMP dans des échantillons de Humain et Souris.

Aperçu rapide pour CDT2/RAMP anticorps (AA 229-256) (ABIN654926)

Antigène: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDT2/RAMP est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: RB24420

Détail du produit anti-CDT2/RAMP anticorps

Épitope: AA 229-256

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.

Isotype: Ig Fraction

Information d'application

Indications d'application: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Détails sur CDT2/RAMP

Antigène: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Autre désignation: DTL

Sujet: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

Poids moléculaire: 79468

ID gène: 51514

NCBI Accession: NP_057532

UniProt: Q9NZJ0