RabbitBP4 / RabbitAP48 (AA 1-425) anticorps

N° du produit ABIN189975

Cet anticorps anti-RabbitBP4 / RabbitAP48 Monoclonal Souris (Clone 15G12) (ABIN189975) détecte spécifiquement RabbitBP4 / RabbitAP48 dans IF/ICC, IHC (p), IP, WB et EMSA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour RabbitBP4 / RabbitAP48 (AA 1-425) anticorps (ABIN189975)

Antigène: RabbitBP4 / RabbitAP48

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (fixed cells) (IF/ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 15G12

Détail du produit

Épitope: AA 1-425

Fonction: Anti-RbAp48 Mouse Monoclonal Antibody

Specificité: This antibody recognizes human and mouse RbAp48 and RbAp46.

Réactivité croisée: Humain

Immunogène: GST fusion protein corresponding to aa 1-425 of human RbAp48.

Isotype: IgG2a

Information d'application

Indications d'application: Immunoblotting: use at 1-5 μg/mL. A band of ~48 kDa is detected. Detection of RbAp48 in K562, THP-1, and HL-60 whole cell extracts (30 μg), separated by 10 % SDS-PAGE, with #3111 at 2 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.
Immunohistochemistry: use at 5-10 μg/mL. Detection of RbAp48 in paraffin-embedded human cervical carcinoma with #3111 at 10 μg/mL.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: This antibody is stable at -20°C for at least one (1) year, store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: RabbitBP4 / RabbitAP48

Sujet: Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110

ID gène: 5925

NCBI Accession: NP_000312

UniProt: P06400