CDC34 anticorps (N-Term)

N° du produit ABIN190280

L’anticorps Souris Monoclonal anti-CDC34 a été validé pour WB, IHC (p), IP et IF/ICC. Il convient pour détecter CDC34 dans des échantillons de Humain.

Aperçu rapide pour CDC34 anticorps (N-Term) (ABIN190280)

Antigène: CDC34 (Cell Division Cycle 34 (CDC34))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDC34 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 14B5

Détail du produit anti-CDC34 anticorps

Épitope: N-Term

Fonction: Anti-cdc34 Mouse Monoclonal Antibody

Specificité: This antibody recognizes human Cdc34.

Réactivité croisée: Humain

Immunogène: N-terminus 6x-His tagged fusion protein encoding full-length human Cdc34 expressed in E. coli.

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting and Immuno-precipitation: use at 1-5 μg/mL.
Positive controls: 293 cells and recombinant fusion protein.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: 10 mM PBS, pH 7.4.

Stock: -20 °C

Stockage commentaire: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Détails sur CDC34

Antigène: CDC34 (Cell Division Cycle 34 (CDC34))

Autre désignation: CDC34

Sujet: Ubiquitin-conjugating enzyme E2 R1,Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421). {PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421, PubMed:22496338}.,Cdc 34 is a ubiquitin conjugating enzyme that is essential for the transition from G1 to S phase in Saccharomyces cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In extracts of Xenopus laevis eggs, Cdc34 is required for the onset of DNA replication and appears to function in a multi-protein complex. Cdc34 is highly conserved among vertebrates and probably functions similarly in all eukaryotes to regulate the start of DNA synthesis.,Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.,EC 2.3.2.23,

UniProt: P49427