Recombinant Histone H1 anticorps

N° du produit ABIN6654274

L’anticorps anti-Histone H1 Monoclonal Souris est utilisé pour la détection de Histone H1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF, FACS et IHC (p).

Aperçu rapide pour Recombinant Histone H1 anticorps (ABIN6654274)

Antigène: Histone H1

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: R1415-1

Détail du produit anti-Histone H1 anticorps

Fonction: Recombinant Histone H1 Antibody

Purification: Protein G affinity chromatography

Immunogène: Nuclei of human leukemia biopsy cells were used as the immunogen for the recombinant Histone H1 antibody.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal dilution of the recombinant Histone H1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
2. View the non-recombinant version of this < a href=../tds/histone-antibody-1415-1-v2567>Histone H1 antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Détails sur Histone H1

Antigène: Histone H1

Sujet: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

UniProt: P07305