POLR2A/RPB1 anticorps (pSer5)

N° du produit ABIN6654646

L’anticorps Souris Monoclonal anti-POLR2A/RPB1 a été validé pour WB, IF et IHC (p). Il convient pour détecter POLR2A/RPB1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour POLR2A/RPB1 anticorps (pSer5) (ABIN6654646)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CTD4H8

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: pSer5

Purification: Purified

Pureté: Protein G affinity chromatography

Immunogène: The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5.

Isotype: IgG1

Information d'application

Indications d'application: Optimal dilution of the recombinant POLR2A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the POLR2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A / RNA polymerase II subunit B1

Sujet: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Pathways: Regulatory RNA Pathways