pan Keratin anticorps

N° du produit ABIN6654982

L’anticorps Lapin Monoclonal anti-pan Keratin a été validé pour IF, FACS, WB et IHC (p). Il convient pour détecter pan Keratin dans des échantillons de Humain, Rat, Souris, Boeuf (Vache), Singe, Porc, Grenouille, Cobaye et Chévre.

Aperçu rapide pour pan Keratin anticorps (ABIN6654982)

Antigène: pan Keratin (panKRT)

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Singe, Porc, Grenouille, Cobaye, Chévre

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp pan Keratin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT-1877R

Détail du produit anti-pan Keratin anticorps

Purification: Purified

Pureté: Protein A affinity chromatography

Immunogène: A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur pan Keratin

Antigène: pan Keratin (panKRT)

Autre désignation: Multi Cytokeratin

Sujet: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.