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NEFL anticorps

L’anticorps Poulet Polyclonal anti-NEFL a été validé pour WB, IF et FM. Il convient pour détecter NEFL dans des échantillons de Boeuf (Vache).
Rockland
N° du produit ABIN6655042
N° du produit (Fournisseur): 200-901-d83

Aperçu rapide pour NEFL anticorps (ABIN6655042)

Antigène

Voir toutes NEFL Anticorps
NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivité

  • 84
  • 52
  • 43
  • 24
  • 22
  • 18
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
Boeuf (Vache)

Hôte

  • 69
  • 35
  • 6
  • 1
  • 1
  • 1
Poulet

Clonalité

  • 67
  • 46
Polyclonal

Conjugué

  • 72
  • 11
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp NEFL est non-conjugé

Application

  • 65
  • 51
  • 29
  • 25
  • 24
  • 19
  • 18
  • 14
  • 14
  • 13
  • 11
  • 8
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • N° du produit (Fournisseur)

    200-901-d83

    Fournisseur

    Rockland

    Fonction

    Neurofilament L Antibody

    Réactivité croisée (Details)

    Anti-NF-L antibody is directed against NF-L protein.

    Purification

    The antibody is a total IgY fraction.

    Immunogène

    Anti-Neurofilament L Antibody was produced by repeated immunizations with preparations of bovine spinal cord intermediate filaments.

    Isotype

    IgG
  • Indications d'application

    IF_Microscopy_Dilution: 1:5000

    Western_Blot_Dilution: 1:10000

    Other: User Optimized

    Commentaires

    Anti-Neurofilament L antibody is tested for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68 kDa in size corresponding to the NF-L proteins in Western blots in the appropriate cell lysate or extract.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    NEFL (Neurofilament, Light Polypeptide (NEFL))

    Autre désignation

    Neurofilament L

    Sujet

    Synonyms: Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

    Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. Anti-Neurofilament L antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.

    Gene Name: NEFL

    ID gène

    4747
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