Rho-related GTP-binding protein anticorps

N° du produit ABIN6655050

N° du produit (Fournisseur): 200-301-g37

Cet anticorps Souris Monoclonal détecte spécifiquement Rho-related GTP-binding protein dans ELISA, WB, IHC, IP, IF et FM. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour Rho-related GTP-binding protein anticorps (ABIN6655050)

Antigène: Rho-related GTP-binding protein (RhO (pan))

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rho-related GTP-binding protein est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 1D4

Détail du produit anti-Rho-related GTP-binding protein anticorps

N° du produit (Fournisseur): 200-301-g37

Fournisseur: Rockland

Fonction: Rhodopsin Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Rhodopsin from all vertebrates based on 100 % homology with the immunizing sequence.

Purification: Anti-Rhodopsin Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Rhodopsin Antibody was produced in mice by repeated immunizations raised against bovine Rhodopsin.

Isotype: IgG1

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Rhodopsin Antibody is tested for use in IF microscopy, IHC, IP, WB, and ELISA. Expect a band approximately ~40kDa corresponding to specific lysates. Binds specifically to the N-terminus of Rhodopsin. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Rho-related GTP-binding protein

Antigène: Rho-related GTP-binding protein (RhO (pan))

Autre désignation: RHO

Sujet:

Synonyms: OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody, Rhodopsin Antibody

Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder.

Gene Name: RHO

ID gène: 509933

NCBI Accession: NP_001014890

UniProt: P02699