GFAP anticorps

N° du produit ABIN6655053

N° du produit (Fournisseur): 200-901-d60

Cet anticorps Poulet Polyclonal détecte spécifiquement GFAP dans WB, IF et FM. Il présente une réactivité envers Boeuf (Vache) et a été mentionné dans 1 publication.

Aperçu rapide pour GFAP anticorps (ABIN6655053)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Boeuf (Vache)

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-GFAP anticorps

N° du produit (Fournisseur): 200-901-d60

Fournisseur: Rockland

Fonction: Glial Fibrillary Acidic Protein (GFAP) Antibody

Réactivité croisée (Details): Anti-GFAP Antibody is directed against bovine GFAP.

Purification: The antibody is a total IgY fraction.

Immunogène: Anti-GFAP Antibody was produced by repeated immunizations with recombinant and extensively purified bovine GFAP.

Isotype: IgG

Information d'application

Indications d'application:

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:10000

Commentaires:

Suggested Applications: IHC
Anti-Glial Fibrillary Acidic Protein (Chicken) antibody is tested for use in Western Blotting, ICC, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 50 kDa in size corresponding to the GFAP proteins in the appropriate cell lysate or extract. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-GFAP (ABIN6655053)

Gleichman, Kawaguchi, Sofroniew, Carmichael: "A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences." dans: Nature communications, Vol. 14, Issue 1, pp. 7426, (2023) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Sujet:

Synonyms: gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345. Glial fibrillary acidic protein, GFAP antibody, neuroscience antibodies

Background: GFAP Antibody detects GFAP. Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers. Therefore, GFAP antibody is ideal for investigators involved in neuropathologic diseases and more generally in Neuroscience.

Gene Name: GFAP

ID gène: 281189

UniProt: Q28115