Cyclin L1 anticorps (AA 314-369)

N° du produit ABIN6655105

Détail du produit anti-Cyclin L1 anticorps

Antigène: Cyclin L1 (CCNL1)

Épitope: AA 314-369

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin L1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96 % homology with the protein from these sourcesCross reactivity with Cyclin L1a homologues from other sources has not been determined.

Purification: The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.

Immunogène:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 10 mg/mL - 20 μg/mL

Application Note: This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry, western blot, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:25,000

Western Blot Dilution: 1:2,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Références pour anticorps anti-Cyclin L1 (ABIN6655105)

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." dans: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Détails sur Cyclin L1

Antigène: Cyclin L1 (CCNL1)

Autre désignation: Cyclin L1 (CCNL1 Produits)

Synonymes: anticorps ANIA6A, anticorps PRO1073, anticorps ania-6a, anticorps 2610030E23Rik, anticorps AU018493, anticorps Ccnl, anticorps Ania-6a, anticorps ccnl1, anticorps fd99b09, anticorps wu:fd99b09, anticorps zgc:55544, anticorps MCM2, anticorps cyclin-L1, anticorps MGC132032, anticorps bm-001, anticorps pro1073, anticorps MGC108436, anticorps Ccnl1, anticorps cyclin L1, anticorps cyclin L1b, anticorps cyclin-L1, anticorps cyclin L1 L homeolog, anticorps cyclin L1 S homeolog, anticorps CCNL1, anticorps Ccnl1, anticorps ccnl1b, anticorps LOC485730, anticorps ccnl1.L, anticorps ccnl1, anticorps LOC100136245, anticorps ccnl1.S

Sujet:

Synonyms: rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL1

ID gène: 57018

NCBI Accession: NP_064703

UniProt: Q9UK58