CD20 anticorps (APC)

N° du produit ABIN6655312

Détail du produit anti-CD20 anticorps (APC)

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD20 est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity with CD20 from other sources has not been tested

Purification: Allophycocyanin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20. Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey. . Anti-CD20 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Immunogène:

Immunogen: Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.

Clone: 2H7

Isotype: IgG2b

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD20 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Références pour anticorps anti-CD20 (APC) (ABIN6655312)

Tedder, Engel: "CD20: a regulator of cell-cycle progression of B lymphocytes." dans: Immunology today, Vol. 15, Issue 9, pp. 450-4, (1994) (PubMed).

Hultin, Hausner, Hultin, Giorgi: "CD20 (pan-B cell) antigen is expressed at a low level on a subpopulation of human T lymphocytes." dans: Cytometry, Vol. 14, Issue 2, pp. 196-204, (1993) (PubMed).

Détails sur CD20

Antigène: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Autre désignation: CD20 (MS4A1 Produits)

Synonymes: anticorps B1, anticorps Bp35, anticorps CD20, anticorps CVID5, anticorps LEU-16, anticorps MS4A2, anticorps S7, anticorps AA960661, anticorps Cd20, anticorps Ly-44, anticorps Ms4a2, anticorps MS4A1, anticorps bp35, anticorps cd20, anticorps ms4a2, anticorps leu-16, anticorps ms4a4c, anticorps cd20-like, anticorps membrane spanning 4-domains A1, anticorps membrane-spanning 4-domains, subfamily A, member 1, anticorps B-lymphocyte antigen CD20, anticorps MS4A1, anticorps Ms4a1, anticorps ms4a1, anticorps LOC101694281

Sujet:

Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

Background: CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 Molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Gene Name: MS4A1

ID gène: 931

NCBI Accession: NP_068769

UniProt: P11836