POLR2A/RPB1 anticorps (pSer2)

N° du produit ABIN6655366

N° du produit (Fournisseur): 200-301-v00

L’anticorps Souris Monoclonal anti-POLR2A/RPB1 a été validé pour WB, IF, ELISA, ChIP et FM. Il convient pour détecter POLR2A/RPB1 dans des échantillons de Humain.

Aperçu rapide pour POLR2A/RPB1 anticorps (pSer2) (ABIN6655366)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: pSer2

N° du produit (Fournisseur): 200-301-v00

Fournisseur: Rockland

Fonction: POL II phospho S2 Antibody

Réactivité croisée (Details): This antibody is specific for Pol II protein phosphorylated Ser2.

Purification: Anti-Pol II pS2 Antibody was purified by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-Pol II S2p Antibody was produced in mice by repeated immunization with the YSPTSPS repeat in the B1 subunit of RNA polymerase II phosphorylated at Ser2 of the repeated sequence.

Isotype: IgG1

Information d'application

Indications d'application:

ChIP_Dilution: 1 μg/ChIP

ELISA_Dilution: 1:3,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Commentaires:

Anti-Pol II pS2 Antibody is tested for Chromatin Immunoprecipitation Sequencing, Chromatin Immunoprecipitation, ELISA, Immunofluorescence and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 225 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: Pol II

Sujet:

Synonyms: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

Background: RNA polymerase II (pol II) is a key enzyme in the regulation and control of gene transcription. It is able to unwind the DNA double helix, synthesize RNA, and proofread the result. Pol II is a complex enzyme, consisting of 12 subunits, of which the B1 subunit (UniProt/Swiss-Prot entry P24928) is the largest. Together with the second largest subunit, B1 forms the catalytic core of the RNA polymerase II transcription machinery. Anti-Pol II S2p Antibody is ideal for research in Gene Expression, Transcription, and Genetics.

Gene Name: POLR2A

ID gène: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatory RNA Pathways