CTSA anticorps

N° du produit ABIN6655368

Détail du produit anti-CTSA anticorps

Antigène: CTSA (Cathepsin A (CTSA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CTSA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: This antiserum is directed against PPCA and reacts with the PPCA from human tissues.  Based on sequence we expect this antibody to react with PPCA from other sources, although specific reactivity has not been confirmed.

Immunogène:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with native 54-kDa human Protective Protein/Cathepsin A (PPCA) precursor, purified from the medium of baculovirus infected insect cells.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500

Application Note: This antibody is suitable for the detection of human PPCA (54-, 32-, and 20- kDa) on Western blot, by immunoprecipitation, immunocytochemistry, and ELISA of either transfected cells (e.g. COS-1 cells) or primary cells (e.g. fibroblasts).

ELISA Dilution: 1:1,000 - 1:5,000

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.1 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur CTSA

Antigène: CTSA (Cathepsin A (CTSA))

Autre désignation: Protective Protein/Cathepsin A (CTSA Produits)

Synonymes: anticorps Ppgb, anticorps beta-galactosidase 2, anticorps PPGB, anticorps GLB2, anticorps GSL, anticorps NGBE, anticorps PPCA, anticorps AU019505, anticorps cathepsin A, anticorps beta-galactosidase 2, anticorps Ctsa, anticorps BGAL2, anticorps CTSA

Sujet:

Synonyms: rabbit anti-Protective Protein Antibody, rabbit anti-Cathepsin A Antibody, Beta Galactosidase Protective Protein antibody, Carboxypeptidase C antibody, Cathepsin A antibody, CTSA antibody, Glactosialidosis antibody, GLB2 antibody, Goldberg Syndrome antibody

Background: Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54- kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20- kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH . Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Gene Name: CTSA, PPGB

ID gène: 5476

UniProt: P10619