CD27 anticorps (FITC)

N° du produit ABIN6655550

Détail du produit anti-CD27 anticorps (FITC)

Antigène: CD27 (CD27 Molecule (CD27))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD27 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Réactivité croisée (Details): Cross reactivity with CD27 from other sources has not been tested

Purification: Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey. . Anti-CD27 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogène:

Immunogen: Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.

Clone: O323

Isotype: IgG1

Labeling Ratio: 4-6

Information d'application

Indications d'application:

Application Note: Anti-CD27 is useful for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Références pour anticorps anti-CD27 (FITC) (ABIN6655550)

Agematsu, Kobata, Sugita, Hirose, Schlossman, Morimoto: "Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).

Hintzen, de Jong, Lens, van Lier: "CD27: marker and mediator of T-cell activation?" dans: Immunology today, Vol. 15, Issue 7, pp. 307-11, (1994) (PubMed).

Détails sur CD27

Antigène: CD27 (CD27 Molecule (CD27))

Autre désignation: CD27 (CD27 Produits)

Synonymes: anticorps TNFRSF7, anticorps S152, anticorps Tnfrsf7, anticorps Tp55, anticorps S152. LPFS2, anticorps T14, anticorps CD27 molecule, anticorps CD27 antigen, anticorps CD27, anticorps Cd27

Sujet:

Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

Background: CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.

Gene Name: CD27

ID gène: 939

NCBI Accession: NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints