HIST1H3A anticorps (H3K27me2)

N° du produit ABIN6655653

N° du produit (Fournisseur): 600-401-v34

Cet anticorps Lapin Polyclonal détecte spécifiquement HIST1H3A dans ELISA, WB, IF, ChIP, DB et FM. Il présente une réactivité envers Humain.

Aperçu rapide pour HIST1H3A anticorps (H3K27me2) (ABIN6655653)

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIST1H3A est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Détail du produit anti-HIST1H3A anticorps

Épitope: H3K27me2

N° du produit (Fournisseur): 600-401-v34

Fournisseur: Rockland

Fonction: Histone H3 K27me2 Antibody

Réactivité croisée (Details): This product reacts with human Histone 3 dimethyl Lys27.

Purification: Anti-H3K27me2 Antibody was purified by affinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-H3K27me2 Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human histone H3, dimethylated at lysine 27.

Isotype: IgG

Information d'application

Indications d'application:

ChIP_Dilution: 2 μg/ChIP

ELISA_Dilution: 1:1,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:50,000

Commentaires:

Anti-H3K27me2 Antibody is tested in Chromatin Immunoprecipitation, Dot Blot, ELISA, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 17 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Agent conservateur: ProClin, Sodium azide

Précaution d'utilisation: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur HIST1H3A

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Autre désignation: HIST1H3A

Sujet:

Synonyms: Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/e, Histone H3/f, Histone H3/g, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called ""histone code"". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Levels of H3K27 dimethylation are higher in silent genes than in active genes suggesting that this histone modification is associated with transcriptional repression. Anti-H3K27me2 Antibody is ideal for research in Chromatin Remodeling, Epigenetics and Gene Expression.

Gene Name: HIST1H3A

ID gène: 3020

NCBI Accession: NP_002098

UniProt: P84243