Tumor Protein p73 anticorps

N° du produit ABIN6655656

Détail du produit anti-Tumor Protein p73 anticorps

Antigène: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Tumor Protein p73 est non-conjugé

Application: Western Blotting (WB)

Purification: Anti-p73 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-p73 from Human and Mouse based on 100% homology with the immunizing sequence. It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73. Because of its reactivity pattern it may be regarded as anti-pan p73. The antibody does not cross react with p53. Cross-reactivity with Anti-p73 from other sources has not been determined.

Immunogène:

Immunogen: p73 Antibody was produced in mice prepared by repeated immunizations with full-length human p73 protein.

Immunogen Type: Native Protein

Clone: 5B1288

Isotype: IgG1

Information d'application

Indications d'application:

Application Note: Anti-p73 Antibody is suitable for use in ChIP, IF/ICC, IP and WB. Expect a band approximately 70 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1-3 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur Tumor Protein p73

Antigène: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Autre désignation: p73 (TP73 Produits)

Synonymes: anticorps P73, anticorps Trp73, anticorps TP73, anticorps tp73, anticorps Tp73, anticorps p73, anticorps TAp73, anticorps zp73theta, anticorps tumor protein p73, anticorps transformation related protein 73, anticorps TP73, anticorps Tp73, anticorps tp73, anticorps Trp73

Sujet:

Synonyms: P73, Tumor protein p73, p53-like transcription factor, p53-related protein

Background: Anti-p73 antibody detects human p73. p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63 % amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein. Tumor BioMarker found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer. Anti-p73 antibody is ideal for investigators involved in cell cycle protein research.

Gene Name: TP73

ID gène: 7161

NCBI Accession: NP_001119712

UniProt: O15350

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus