Cyclin L2 anticorps (AA 309-384)

N° du produit ABIN6655712

Détail du produit anti-Cyclin L2 anticorps

Antigène: Cyclin L2 (CCNL2)

Épitope: AA 309-384

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cyclin L2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée: Humain, Souris

Réactivité croisée (Details): Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80 % homology with the protein from these sourcesCross reactivity with Cyclin L2a homologues from other sources has not been determined.

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using polypeptide coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This antibody is specific for human Cyclin L2a and shows negligible reactivity to Cyclin L2b or Cyclin L1 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L2a protein from rat and chicken (called Cyclin S) is expected, as this sequence shows ~ 80% homology with the protein from these sources. Cross reactivity with Cyclin L2a homologues from other sources has not been determined.

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to amino acids 309-384 of Human Cyclin L2a protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 58 kDa in size corresponding to Cyclin L2a by western blotting in the appropriate cell lysate or extract. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation.

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:3,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Références pour anticorps anti-Cyclin L2 (ABIN6655712)

Kren, Unger, Abedin, Vogel, Henzler, Ahmed, Trembley: "Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy." dans: Breast cancer research : BCR, Vol. 17, pp. 19, (2015) (PubMed).

Loyer, Trembley, Katona, Kidd, Lahti: "Role of CDK/cyclin complexes in transcription and RNA splicing." dans: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).

Détails sur Cyclin L2

Antigène: Cyclin L2 (CCNL2)

Autre désignation: Cyclin L2 (CCNL2 Produits)

Synonymes: anticorps CCNL2, anticorps ANIA-6B, anticorps CCNM, anticorps HCLA-ISO, anticorps HLA-ISO, anticorps PCEE, anticorps SB138, anticorps CCNL1, anticorps 1700010A01Rik, anticorps 1810019L15Rik, anticorps 2010319M22Rik, anticorps AA409936, anticorps AW261738, anticorps Ania-6b, anticorps HCLA-150, anticorps Pcee, anticorps cyclin L2, anticorps cyclin L2 L homeolog, anticorps CCNL2, anticorps ccnl2.L, anticorps Ccnl2

Sujet:

Synonyms: rabbit anti-Cyclin-L2 antibody, Cyclin L2, CCNL2, SB138, Paneth cell-enhanced expression protein, Cyclin Ania-6b, Paneth cell-enhanced expression protein, PCEE, Ania6b

Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively).  Cyclin L2 can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). The Cyclin L2 gene is located approximately 100-200 kb from the PITSLRE/CDK11 protein kinase locus in human (1p36) and in a syngenetic location on mouse chromosome 4q.  Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Gene Name: CCNL2, SB138

ID gène: 81669, 14585859

UniProt: Q96S94