EZH2 anticorps (N-Term)

N° du produit ABIN6656040

N° du produit (Fournisseur): 200-401-w53

Cet anticorps Lapin Polyclonal détecte spécifiquement EZH2 dans WB, IHC, IF et ChIP. Il présente une réactivité envers Humain.

Aperçu rapide pour EZH2 anticorps (N-Term) (ABIN6656040)

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EZH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-EZH2 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 200-401-w53

Fournisseur: Rockland

Fonction: EzH2 Antibody

Réactivité croisée (Details): Cross reactivity with other species was not tested.

Purification: Anti-EZH2 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.

Isotype: IgG

Information d'application

Indications d'application:

ChIP_Dilution: 2.5 μg per IP

Immunohistochemistry_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:1,000

Commentaires:

Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Agent conservateur: ProClin, Sodium azide

Précaution d'utilisation: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur EZH2

Antigène: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Autre désignation: EzH2

Sujet:

Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2

Background: EZH2 is a histone-lysine methyltransferase which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.

Gene Name: EZH2

ID gène: 2146

NCBI Accession: NP_001190176

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation