Vimentin anticorps

N° du produit ABIN6656056

N° du produit (Fournisseur): 200-301-e54

L’anticorps anti-Vimentin Monoclonal Souris est utilisé pour la détection de Vimentin dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA et FM.

Aperçu rapide pour Vimentin anticorps (ABIN6656056)

Antigène: Vimentin (VIM)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Clone: 2D1

Détail du produit anti-Vimentin anticorps

N° du produit (Fournisseur): 200-301-e54

Fournisseur: Rockland

Fonction: Vimentin Antibody

Purification: Anti-Vimentin Antibody was purified from tissue culture supernatant by protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-Vimentin Antibody was produced in mice by repeated immunizations with a recombinant human vimentin purified from E. coli.

Isotype: IgG2a

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500-1:1000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Other: ICC: 1:500-1:2000

Commentaires:

Anti-Vimentin Antibody is tested for Western Blot, immunocytochemistry, and immunohistochemistry, and it is specific for the ~50 kDa vimentin protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Date de péremption: 12 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet:

Synonyms: Vim, Vimentin Antibody

Background: Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin Antibody is ideal for researchers interested in neuroscience research.

Gene Name: VIM

ID gène: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis