CRYAA anticorps

N° du produit ABIN6656059

N° du produit (Fournisseur): 200-301-e84

L’anticorps Souris Monoclonal anti-CRYAA a été validé pour WB, ELISA et IF. Il convient pour détecter CRYAA dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour CRYAA anticorps (ABIN6656059)

Antigène: CRYAA (Crystallin, alpha A (CRYAA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CRYAA est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 1H3-B8

Détail du produit anti-CRYAA anticorps

N° du produit (Fournisseur): 200-301-e84

Fournisseur: Rockland

Fonction: Alpha A Crystallin Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Alpha A Crystallin from Human, Mouse, Cow, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Alpha A Crystallin Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Alpha Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha Crystallin protein.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Commentaires:

Anti-Alpha A Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa protein corresponding to the molecular mass of α-crystallin on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-CRYAA (ABIN6656059)

Wei, Hao, Chen, Gan, Fan: "A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation." dans: Experimental eye research, Vol. 226, pp. 109306, (2023) (PubMed).

Détails sur CRYAA

Antigène: CRYAA (Crystallin, alpha A (CRYAA))

Autre désignation: Alpha A Crystallin

Sujet:

Synonyms: Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4, Alpha-crystallin A chain

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases.

Gene Name: CRYAA

ID gène: 1409

NCBI Accession: NP_000385

UniProt: P02489

Pathways: M Phase