FKBP4 anticorps

N° du produit ABIN6656074

N° du produit (Fournisseur): 200-301-f29

L’anticorps Souris Monoclonal anti-FKBP4 a été validé pour WB, IHC, IP et IF. Il convient pour détecter FKBP4 dans des échantillons de Humain.

Aperçu rapide pour FKBP4 anticorps (ABIN6656074)

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FKBP4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: Hi52C

Détail du produit anti-FKBP4 anticorps

N° du produit (Fournisseur): 200-301-f29

Fournisseur: Rockland

Fonction: FKBP52 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with FKBP52 from Canine, Hamster, Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-FKBP52 Antibody was purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: FKBP51 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the residues of human FKBP51.

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: 5 μg/20uL

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:2000

Commentaires:

Anti-FKBP52 Antibody is tested for WB, IP, IF, and IHC. Expect a band approximately ~52kDa protein representing FKBP52 in cell lysates. Heavy chain migrates close to FKBP52 on SDS PAGE. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur FKBP4

Antigène: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Autre désignation: FKBP52

Sujet:

Synonyms: FK506 binding protein 4, FKBP4, FKBP59, HBI, Hsp56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein, FKBP52

Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics . FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.

Gene Name: FKBP4

ID gène: 2288

NCBI Accession: NP_002005

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway