GFAP anticorps (AA 411-422)

N° du produit ABIN6656791

N° du produit (Fournisseur): 200-301-w55

L’anticorps Souris Monoclonal anti-GFAP a été validé pour WB, IHC, FM et MA. Il convient pour détecter GFAP dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour GFAP anticorps (AA 411-422) (ABIN6656791)

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: S206A-8

Détail du produit anti-GFAP anticorps

Épitope: AA 411-422

N° du produit (Fournisseur): 200-301-w55

Fournisseur: Rockland

Fonction: GFAP Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-GFAP Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-GFAP Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-GFAP Antibody is tested for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-GFAP (ABIN6656791)

Rodrigues-Amorim, Rivera-Baltanás, Del Carmen Vallejo-Curto, Rodriguez-Jamardo, de Las Heras, Barreiro-Villar, Blanco-Formoso, Fernández-Palleiro, Álvarez-Ariza, López, García-Caballero, Olivares, Spuch: "Plasma β-III tubulin, neurofilament light chain and glial fibrillary acidic protein are associated with neurodegeneration and progression in schizophrenia." dans: Scientific reports, Vol. 10, Issue 1, pp. 14271, (2021) (PubMed).

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP

Sujet:

Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

Gene Name: GFAP

ID gène: 2670

UniProt: P14136