EEF1A2 anticorps (pSer358)

N° du produit ABIN6657445

N° du produit (Fournisseur): 600-401-x05

L’anticorps Lapin Polyclonal anti-EEF1A2 a été validé pour WB et ELISA. Il convient pour détecter EEF1A2 dans des échantillons de Humain.

Aperçu rapide pour EEF1A2 anticorps (pSer358) (ABIN6657445)

Antigène: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EEF1A2 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-EEF1A2 anticorps

Épitope: pSer358

N° du produit (Fournisseur): 600-401-x05

Fournisseur: Rockland

Fonction: eEF1A2 Phospho S358 Antibody

Réactivité croisée (Details): Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Phospho-Ser358 eEF1A2 Antibody has been tested by Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Date de péremption: 12 months

Détails sur EEF1A2

Antigène: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Autre désignation: eEF1A2

Sujet:

Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1

Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.

Gene Name: EEF1A2

ID gène: 1917

NCBI Accession: NP_001949

UniProt: Q05639