EEF1A2 anticorps (pSer358)

N° du produit ABIN6657445

Détail du produit anti-EEF1A2 anticorps

Antigène: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Épitope: pSer358

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EEF1A2 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Souris

Purification: Anti-Phospho-Ser358 eEF1A2 Antibody is directed against human eEF1A2 protein phosphorylated at S358. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated eEF1A2. Minimal reactivity occurs against non-phosphorylated eEF1A2. Reactivity against eEF1A2 occurs from mouse sources. However, reactivity is also expected against chicken, human, non-human primate, rat, sheep, and Xenopus based on 100% sequence homology.

Immunogène:

Immunogen: Anti-Phospho-Ser358 eEF1A2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 of human eEF1A2 conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Phospho-Ser358 eEF1A2 Antibody is suitable for Western Blots and is specific for the ~50 kDa eEF1A2 protein phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein). Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Agent conservateur: Without preservative

Stock: RT,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Détails sur EEF1A2

Antigène: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Autre désignation: eEF1A2 (EEF1A2 Produits)

Synonymes: anticorps EEF1AL, anticorps EF-1-alpha-2, anticorps EF1A, anticorps HS1, anticorps STN, anticorps STNL, anticorps EEF1A2, anticorps Eef1a, anticorps S1, anticorps wasted, anticorps wst, anticorps Ps10, anticorps RATPS10, anticorps Stnl, anticorps eef1al, anticorps ef1a, anticorps stnl, anticorps EEF1A-2, anticorps zgc:92085, anticorps eukaryotic translation elongation factor 1 alpha 2, anticorps eukaryotic translation elongation factor 1 alpha 1, anticorps eukaryotic translation elongation factor 1 alpha 2 S homeolog, anticorps putative elongation factor 1-alpha-like 3, anticorps EEF1A2, anticorps EEF1A1, anticorps Eef1a2, anticorps eef1a2.S, anticorps eef1a2, anticorps LOC739210, anticorps EEF1, anticorps eef1

Sujet:

Synonyms: elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1

Background: Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle. Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors. Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites. Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome. eEF1A2 pS358 Antibody is ideal for researchers interested in cancer research.

Gene Name: EEF1A2

ID gène: 1917

NCBI Accession: NP_001949

UniProt: Q05639