Nitrotyrosine anticorps

N° du produit ABIN6657486

N° du produit (Fournisseur): 200-301-a98

Cet anticorps Souris Monoclonal détecte spécifiquement Nitrotyrosine dans WB, ELISA et IHC. Il présente une réactivité envers .

Aperçu rapide pour Nitrotyrosine anticorps (ABIN6657486)

Antigène: Nitrotyrosine

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nitrotyrosine est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 39B6

Détail du produit anti-Nitrotyrosine anticorps

N° du produit (Fournisseur): 200-301-a98

Fournisseur: Rockland

Fonction: Nitrotyrosine Antibody

Réactivité croisée (Details): This antibody is known to react with nitrated tyrosine residues from human, mouse, rat and dog sources.

Purification: This Protein G purified monoclonal antibody reacts with proteins containing 3-nitrotyrosine moieties and does not detect non-nitrated tyrosine residues.

Stérilité: Sterile filtered

Immunogène: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid - BSA conjugate.

Isotype: IgG2a

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:200 - 1:1,000

Western_Blot_Dilution: 1:500 - 1:2,000

Other: User Optimized

Commentaires:

This Protein G purified antibody has been tested for use in ELISA, immunoprecipitation, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Approximately 0.7 μg of antibody is sufficient to detect 5 μg of SIN-1 treated BSA by Western blotting using HRP Gt-a-Mouse IgG (p/n 610-103-121) and chemiluminescent substrate (p/n PICOMAX-110).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Nitrotyrosine

Antigène: Nitrotyrosine

Classe de substances: Chemical

Sujet:

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation-induced tissue injury (1). The presence of nitro tyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Gene Name: n/a