MYOD1 anticorps (Internal Region)

N° du produit ABIN6657593

N° du produit (Fournisseur): 200-301-h94

Cet anticorps anti-MYOD1 est un anticorps Souris Monoclonal détectant MYOD1 dans WB, IF, IHC, FACS et IP. Adapté pour Souris.

Aperçu rapide pour MYOD1 anticorps (Internal Region) (ABIN6657593)

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 5-8A

Détail du produit anti-MYOD1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 200-301-h94

Fournisseur: Rockland

Fonction: MyoD Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-MyoD Antibody was purified by Protein G chromatography.

Immunogène: MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: 1 μg/mL

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1 μg/mL

Commentaires:

Anti-MyoD Antibody is tested for use in WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, and IP. Expect a band approximately 45kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.02 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD

Sujet:

Synonyms: Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3

Background: Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.

Gene Name: Myod1

ID gène: 17927

NCBI Accession: NP_034996

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development