MYOD1 anticorps (Internal Region)

N° du produit ABIN6657593

Détail du produit anti-MYOD1 anticorps

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Épitope: Internal Region

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MYOD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Réactivité croisée: Humain

Purification: Anti-MyoD Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-MyoD from Human, Cat, Rat, and Mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-MyoD from other sources has not been determined.

Immunogène:

Immunogen: MyoD Antibody was produced in mice prepared by repeated immunizations with recombinant mouse MyoD protein at an internal amino acid sequence.

Immunogen Type: Recombinant Protein

Clone: 5-8A

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-MyoD Antibody is suitable for use in IF/ICC, IHC (frozen), IP and WB. Expect a band approximately 45 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: 1 μg/mL

Western Blot Dilution: 1 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.02 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur MYOD1

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: MyoD (MYOD1 Produits)

Synonymes: anticorps MYF3, anticorps MYOD, anticorps PUM, anticorps bHLHc1, anticorps MyoD, anticorps XMyoD, anticorps XtmyoD, anticorps mf25, anticorps myf3, anticorps pum, anticorps MUF3, anticorps AI503393, anticorps Myod-1, anticorps etID309723.25, anticorps myod, anticorps wu:fb57a01, anticorps zgc:136744, anticorps MYOD1, anticorps myod1, anticorps mf1, anticorps myod1-a, anticorps myod1-b, anticorps myogenic differentiation 1, anticorps MYOD protein, anticorps myogenic factor MyoD1, anticorps myogenic differentiation 1 S homeolog, anticorps myogenic differentiation 1 L homeolog, anticorps MYOD1, anticorps myod1, anticorps Myod1, anticorps myod, anticorps myod1.S, anticorps myod1.L

Sujet:

Synonyms: Myod, Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, bHLHc1, Myogenic factor 3, Myf-3

Background: Anti-MyoD Antibody detects MyoD. The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are: myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin). Anti-MyoD Antibody is ideal for investigators involved in cell cycle protein, cytokines and growth factors.

Gene Name: Myod1

ID gène: 17927

NCBI Accession: NP_034996

UniProt: P10085

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development