BAT3 anticorps (C-Term)

N° du produit ABIN6657747

N° du produit (Fournisseur): 100-401-b63s

Cet anticorps Lapin Polyclonal détecte spécifiquement BAT3 dans WB et ELISA. Il présente une réactivité envers Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour BAT3 anticorps (C-Term) (ABIN6657747)

Antigène: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BAT3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-BAT3 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 100-401-b63s

Fournisseur: Rockland

Fonction: Scythe/Bat3 Antibody

Purification: This product was prepared from monospecific antiserum by addition of Sodium azide to 0.01 % .

Stérilité: Sterile filtered

Immunogène: This Bat3 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/Bat3.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: User Optimized

Western_Blot_Dilution: 1:5,000 to 1:8,000

Commentaires:

This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approximately 200kDa in size corresponding to Scythe/BAT3 by western blotting in the appropriate cell lysate or extract. In western blot analysis of endogenous protein, multiple bands of Scythe/BAT3 may appear corresponding to variable amount of splice isoforms present.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 3 months

Références pour anticorps anti-BAT3 (ABIN6657747)

Desmots, Russell, Michel, McKinnon: "Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis." dans: The Journal of biological chemistry, Vol. 283, Issue 6, pp. 3264-71, (2008) (PubMed).

Desmots, Russell, Lee, Boyd, McKinnon: "The reaper-binding protein scythe modulates apoptosis and proliferation during mammalian development." dans: Molecular and cellular biology, Vol. 25, Issue 23, pp. 10329-37, (2005) (PubMed).

Détails sur BAT3

Antigène: BAT3 (Large Proline-Rich Protein BAT3 (BAT3))

Autre désignation: Scythe/Bat3

Sujet:

Synonyms: rabbit anti-Bat3 Antibody, rabbit anti-Scythe Antibody, Bat3, HLA-B-associated transcript 3, Large proline-rich protein BAT3

Background: Scythe protein (also known as Bat3 and HLA-B-associated transcript 3) is an apoptotic regulator that is highly conserved in eukaryotes and contains a ubiquitin-like domain near its N-terminus. Scythe binds Reaper, a potent apoptotic inducer. Scythe and Reaper are thought to signal apoptosis, in part through regulating the folding and and activity of apoptotic signaling molecules. Scythe regulates apoptosis-inducing factor stability during endoplasmic reticulum stress-induced apoptosis. Scythe interacts with transforming growth factor-beta (TGF-beta) receptors and enhances TGF-beta1-induced type I collagen expression in mesangial cells. Scythe and SET1A form a complex with CTCFL/BORIS to modulate H3K4 histone dimethylation and gene expression. HLA-B-associated transcript 3 (Bat3/Scythe) is essential for p300-mediated acetylation of p53. Bat3 is identified as a critical regulator of Hsp70-2 in spermatogenesis, thereby providing a possible molecular target in idiopathic male infertility. The human Scythe/BAT3 ortholog in rodents is predominantly and developmentally expressed in testis.

Gene Name: BAT3

ID gène: 224727, 33147082

UniProt: Q9Z1R2