TNNI3 anticorps (pSer150)

N° du produit ABIN6658032

N° du produit (Fournisseur): 600-401-x33

Cet anticorps Lapin Polyclonal détecte spécifiquement TNNI3 dans WB et ELISA. Il présente une réactivité envers Souris.

Aperçu rapide pour TNNI3 anticorps (pSer150) (ABIN6658032)

Antigène: TNNI3 (Cardiac Troponin I (TNNI3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNNI3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-TNNI3 anticorps

Épitope: pSer150

N° du produit (Fournisseur): 600-401-x33

Fournisseur: Rockland

Fonction: Troponin I (cardiac) Phospho S150 Antibody

Réactivité croisée (Details): Anti-Phospho-Ser150 Troponin (cardiac) Antibody is directed against Troponin protein phosphorylated at S150.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Anti-Phospho-Ser150 Troponin (cardiac) Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 pf cardiac troponin I conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-Phospho-Ser150 Troponin (cardiac) Antibody has been tested in Western Blots and is specific for the ~25 kDa cardiac troponin I protein phosphorylated at Ser 150. Immunolabeling is greatly decreased with lambda-phosphatase treatment. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Date de péremption: 12 months

Détails sur TNNI3

Antigène: TNNI3 (Cardiac Troponin I (TNNI3))

Autre désignation: Troponin I (cardiac)

Sujet:

Synonyms: Troponin I cardiac muscle, Cardiac troponin I

Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 and is phosphorylated by AMPK at Ser23 and Ser150. Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI. Troponin I pS150 Antibody is ideal for researchers interested in cancer research.

Gene Name: TNNI3

ID gène: 21954

NCBI Accession: NP_033432

UniProt: P48787